Journal: International Journal of Molecular Sciences

Article Title: Nrf2 Deficiency Exacerbates the Decline in Swallowing and Respiratory Muscle Mass and Function in Mice with Aspiration Pneumonia

doi: 10.3390/ijms252111829

Figure Lengend Snippet: ( A – G ): The changes in body weight and lung pathology in challenged mice. ( A ): The body weight change during the 28-day aspiration challenge. * p < 0.05 vs. wild-types. ( B – G ) : The lung tissue sections that are representative of controls (( B ): wild-types, ( E ): Nrf2 -knockouts), and after 28 days of aspiration challenge (( C , D ): wild-types, ( F , G ): Nrf2 -knockouts) stained for hematoxylin and eosin. ( H – O ): Confocal images of leucocytes infiltrating into the lungs in wild-type ( H – K ) or Nrf2 -knockout ( L – O ) mice after a 28-day challenge ( I , K , M , O ) or in controls ( H , J , L , N ). A few S100A8-immunoreactive neutrophils (green) and F4/80-immunoreactive macrophages (red) infiltrated the controls ( H , L ). Many neutrophils and macrophages infiltrated the challenged lungs ( I , M ). We recognized a similar trend in the infiltrations of CD3e-immunoreactive T cells (green) and B220-immunoreactive B cells (red) ( J , K , N , O ). ( P – S ): The area densities of infiltrated leukocytes were differentially quantified. Macrophages and B cells infiltrated more in Nrf2 -knockouts than in wild-types in challenged groups ( Q , S ). Scale bars: ( B , C , E , F ): 500 µm in ( B ). ( D , G ): 200 µm in ( D ). ( H – O ): 50 µm in ( H ). * p below 0.05 versus controls, † p below 0.05 versus challenged wild-types. Five mice per group; data are from 2 individual experiments.

Article Snippet: The dilution and antibodies used primarily for immunohistochemistry were as follows ( ): neutrophils: S100 calcium-binding protein A8 (S100A8; 1:500; goat; AF3059, R&D Systems, Minneapolis, MN, USA); macrophages: F4/80 (1:100; Rat; MCA497G, Bio-Rad, UK); T cells: CD3e (1:500; hamster; eBio500A2, eBioscience, San Diego, CA, USA); B cells: B220/CD45R (1:500; rat; RA3-6B2, eBioscience).

Techniques: Staining, Knock-Out

Journal: Scientific Reports

Article Title: Plasma S100A8/A9 level predicts response to immune checkpoint inhibitors in patients with advanced non-small cell lung cancer

doi: 10.1038/s41598-025-87232-z

Figure Lengend Snippet: Laboratory blood test results in all patients ( n = 81).

Article Snippet: A mouse anti-human S100A8/A9 antibody (clone #260) was immobilized on a 96-well MaxiSorp ELISA plate (Thermo Fisher Scientific, Waltham, MA, USA) by incubating overnight at 4 °C.

Techniques:

Journal: Scientific Reports

Article Title: Plasma S100A8/A9 level predicts response to immune checkpoint inhibitors in patients with advanced non-small cell lung cancer

doi: 10.1038/s41598-025-87232-z

Figure Lengend Snippet: Patient characteristics and laboratory findings of high and low S100A8/A9 groups.

Article Snippet: A mouse anti-human S100A8/A9 antibody (clone #260) was immobilized on a 96-well MaxiSorp ELISA plate (Thermo Fisher Scientific, Waltham, MA, USA) by incubating overnight at 4 °C.

Techniques:

Journal: Scientific Reports

Article Title: Plasma S100A8/A9 level predicts response to immune checkpoint inhibitors in patients with advanced non-small cell lung cancer

doi: 10.1038/s41598-025-87232-z

Figure Lengend Snippet: Kaplan–Meier curves representing time to treatment failure for immune checkpoint inhibitor therapy ( a ) and overall survival ( b ) in plasma S100A8/A9 low (dashed line, n = 47) and high groups (solid line, n = 34). CI confidence interval, NE not estimable, OS overall survival, TTF time to treatment failure.

Article Snippet: A mouse anti-human S100A8/A9 antibody (clone #260) was immobilized on a 96-well MaxiSorp ELISA plate (Thermo Fisher Scientific, Waltham, MA, USA) by incubating overnight at 4 °C.

Techniques:

Journal: Scientific Reports

Article Title: Plasma S100A8/A9 level predicts response to immune checkpoint inhibitors in patients with advanced non-small cell lung cancer

doi: 10.1038/s41598-025-87232-z

Figure Lengend Snippet: Univariate and multivariate analyses of the factors associated with the TTF of immunotherapies.

Article Snippet: A mouse anti-human S100A8/A9 antibody (clone #260) was immobilized on a 96-well MaxiSorp ELISA plate (Thermo Fisher Scientific, Waltham, MA, USA) by incubating overnight at 4 °C.

Techniques:

Journal: Scientific Reports

Article Title: Plasma S100A8/A9 level predicts response to immune checkpoint inhibitors in patients with advanced non-small cell lung cancer

doi: 10.1038/s41598-025-87232-z

Figure Lengend Snippet: Univariate and multivariate analyses of the factors associated with the OS.

Article Snippet: A mouse anti-human S100A8/A9 antibody (clone #260) was immobilized on a 96-well MaxiSorp ELISA plate (Thermo Fisher Scientific, Waltham, MA, USA) by incubating overnight at 4 °C.

Techniques:

Journal: Scientific Reports

Article Title: Plasma S100A8/A9 level predicts response to immune checkpoint inhibitors in patients with advanced non-small cell lung cancer

doi: 10.1038/s41598-025-87232-z

Figure Lengend Snippet: Kaplan–Meier curves representing time to treatment failure for immune checkpoint inhibitor therapy ( a ) and overall survival ( b ) in plasma S100A8/A9 low (dashed line, n = 12) and high groups (solid line, n = 11) with programmed death ligand 1 expression < 1%. CI confidence interval, NE not estimable, OS overall survival, TTF time to treatment failure, PD-L1 programmed death -ligand 1.

Article Snippet: A mouse anti-human S100A8/A9 antibody (clone #260) was immobilized on a 96-well MaxiSorp ELISA plate (Thermo Fisher Scientific, Waltham, MA, USA) by incubating overnight at 4 °C.

Techniques: Expressing

Journal: Journal of Translational Internal Medicine

Article Title: Identification of functional heterogeneity of immune cells and tubular-immune cellular interplay action in diabetic kidney disease

doi: 10.2478/jtim-2023-0130

Figure Lengend Snippet: Characterization of mice and validation of macrophage after 8 weeks of treatments. (A) Schematics of the experimental design. UAE were monitored in 8-week-old db/db mice. Treatment (PBS, SGLT2i) was started after db/db mice developed DKD approximately 10 weeks of age. All mice were sacrificed 8 weeks after treatment, Four mice per group. (B) Urinary albumin excretion (UAE) in db/mc, db/db and db/db+SGLT2i group. (C) Kidney sections from each group were stained with PAS. Scale bars, 20 μm. (D) Kidney samples of each group were stained with the S100a8 (green), S100a9 (green) and M2 macrophage (CD206, red). Scale bars, 20 μm. The data are the means ± SD. One-way ANOVA was performed followed by Dunnett’s multiple comparisons. *** P < 0.001.

Article Snippet: The antibodies and reagents used in this study included rabbit anti-CD206 (ab64693, 1:400, Abcam, Cambridge, UK), goat anti-S100a8 (AF3059, 1:100, R&D systems, MN, USA), goat anti-S100a9 (1:100, AF2065, R&D systems, MN, USA), DNA was stained with Hoechst 33342 (1:1000, H3570; Thermo Fisher Scientific, CA, USA), dapagliflozin (3 mg/kg/d, S1548, Selleck, TX, USA)

Techniques: Staining